@article{Ishrath Razia Riaz_Anusha Hindupur_Thota Akhil Raj_Iswarya Anbazhagan_Prabu Dhandapani_2022, title={Detection of Colistin Resistance in Uropathogenic Carbapenem Resistant Klebsiella pneumoniae}, volume={9}, url={https://apjhs.com/index.php/apjhs/article/view/1822}, DOI={10.21276/apjhs.2022.9.1.45}, abstractNote={<p>Background: Colistin resistance among carbapenem-resistant Klebsiella pneumoniae (CRKp) strains at an accelerated rate poses a serious global threat which limits therapeutic options. Objective: In this study, 203 laboratory-archived uropathogenic K. pneumoniae isolates were screened for carbapenem and colistin resistance. Methods: Carbapenam resistance screening was performed using combined disc test. Colistin resistance was determined for CRKp isolates, according to clinical and laboratory standards institute- European Committee on Antimicrobial Susceptibility Testing guidelines. Polymerase Chain Reaction for carbapenamase genes (blaNDM-1, blaKPC-2 and blaOXA-1) and plasmid-mediated colistin resistance genes (mcr-1-2) were performed for the resistant isolates. Results: About 30% of the CRKp isolates were resistant to colistin; 57.1% and 35.7% of the isolates carried blaNDM-1 and blaOXA-1 gene, respectively. None of the isolates showed positive for blaKPC-2 and mcr (1-2) genes. Conclusion: Colistin resistance in the absence of a plasmid borne transfer mechanism was observed among our CRKp strains. Escalating colistin resistance is a crucial obstacle in treatment of K. pneumoniae infections and repurposing their usage is important to combat and control the resistance.</p>}, number={1}, journal={Asian Pacific Journal of Health Sciences}, author={Ishrath Razia Riaz and Anusha Hindupur and Thota Akhil Raj and Iswarya Anbazhagan and Prabu Dhandapani}, year={2022}, month={Jan.}, pages={156–157} }