Intestinal sepsis model of small animals
DOI:
https://doi.org/10.21276/apjhs.2020.7.3.11Abstract
Objective: The goal of this paper was to develop a model of intestinal sepsis in the investigational animal.
Material and methods. Sprague-Dawley male rats of 4 weeks (N = 42) were used. Initially, a pilot study (N = 12) was performed and distributed in groups with .8 cc inoculum of Escherichia coli ATCC intraperitoneally administered at concentrations of 9, 7, 6. Subsequently, concentrations of 9 CFU are used in two groups of rats with reductions of 11 cc. Finally, a randomized trial of 28 rats was initiated in three treatment groups after intraperitoneal infection. Biological models of blood and peritoneal fluid were observed, and histopathological study of intraperitoneal tissues was done.
Results. Demise of 95% of the rats infected with E. coli UFC concentration was observed. The blood culture and peritoneal fluid culture was positive for the same strain in all of them. The formation of blisters on the liver surface and polymorphonuclear infiltration in organs were detected.
Conclusion. The fatal dose of E. coli should be diluted for intraperitoneal injection.
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References
Remick DG, Ward PA. Evaluation of endotoxin models for the study of sepsis. Shock 2005;24:7-11.
Gomez-Jimenez J, Salgado A, Mourelle M, Martin MC, Segura RM, Peracaula R, et al. L-arginine: Nitric oxide pathway in endotoxemia and human septic shock. Crit Care Med 1995;23:253-8.
Dejager L, Pinheiro I, Dejonckheere E, Libert C. Cecal ligation and puncture: The gold standard model for polymicrobial sepsis? Trends Microbiol 2011;19:198-208.
Belga S, Chiang D, Kabbani D, Abraldes JG, Cervera C. The direct and indirect effects of vancomycin-resistant enterococci colonization in liver transplant candidates and recipients. Expert Rev Anti Infect Ther 2019;17:363-73.
Garibaldi RA, Allman GW, Larsen DH, Smith CB, Burke JP. Detection of endotoxemia by the limulus test in patients with indwelling urinary catheters. J Infect Dis 1973;128:551-4.
Edmiston CE, Goheen MP, Kornhall S, Jones FE, Condon RE. Fecal peritonitis: Microbial adherence to serosal mesothelium and resistance to peritoneal lavage. World J Surg 1990;14:176-83.
Wang P, Chaudry IH. A single hit model of polymicrobial sepsis: Cecal ligation and puncture. Sepsis 1998;2:227-33.
Doi K, Leelahavanichkul A, Yuen PS, Star RA. Animal models of sepsis and sepsis-induced kidney injury. J Clin Invest 2009;119:2868-78.
Kuczmarska-Haas A, Yadav P, Musunuru HB, Witt JS, Francis DM, Rosenberg SA, et al. Assessing radiation induced liver damage following MR-guided stereotactic body radiation therapy (SBRT): Challenging current dose constraints. Int J Radiat Oncol Biol Phys 2018;102:e25.
Brealey D, Karyampudi S, Jacques TS, Novelli M, Stidwill R, Taylor V, et al. Mitochondrial dysfunction in a long-term rodent model of sepsis and organ failure. Am J Physiol Regul Integr Comp Physiol 2004;286:R491-7.
Schabbauer G. Polymicrobial sepsis models: CLP versus CASP. Drug Discov Today Dis Models 2012;9:e17-21.
Bauer TM, Fernández J, Navasa M, Vila J, Rodés J. Failure of Lactobacillusspp. to prevent bacterial translocation in a rat model of experimental cirrhosis. J Hepatol 2002;36:501-6.
Sammour T, Kahokehr A, Soop M, Hill AG. Peritoneal damage: The inflammatory response and clinical implications of the neuro-immuno-humoral axis. World J Surg 2010;34:704-20.
Osuchowski MF, Ayala A, Bahrami S, Bauer M, Boros M, Cavaillon JM, et al. Minimum quality threshold in pre-clinical sepsis studies (MQTiPSS): An international expert consensus initiative for improvement of animal modeling in sepsis. Intensive Care Med Exp 2018;6:26.
Garrido AG, Figueiredo LF. Experimental models of sepsis and septic shock: An overview. Acta Cir Bras 2004;19:82-8.
Unsinger J, McDonough JS, Shultz LD, Ferguson TA, Hotchkiss RS. Sepsis‐induced human lymphocyte apoptosis and cytokine production in humanized mice. J Leukoc Biol 2009;86:219-27.
Brehm MA, Shultz LD, Greiner DL. Humanized mouse models to study human diseases. Curr Opin Endocrinol Diabetes Obes 2010;17:120.
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