Saliva as the Stress Biomarker after Fasting Exposure on Adult Girls and Boys
Keywords:antioxidant, DPPH, FRAP, fasting, saliva
This experiment was conducted to determine the fasting effects on anthropometric parameters and salivary antioxidant properties in girls and boys. Saliva samples were collected from eighteen healthy girls (control-09 & fasting-09 girls) and twelve healthy boys (control-06 & fasting-06 boys). Saliva was collected four times from all the subjects at a time interval of two hours. For anthropometric parameters, body weight, body temperature, pulse rate (PR), systolic blood pressure (SBP), diastolic blood pressure (DBP) was measured during each saliva collection time. Antioxidant parameters of all the saliva samples were evaluated. Most of the anthropometric parameters like temperature, PR, SBP, and DBP of the fasting group was significantly (p<0.5) decreases than the control group of both boy's and girl's in the different time interval. Antioxidant properties were significantly (p<0.5) higher in the fasting group of girls, but the insignificantly different level was found in the boy's group. This study was indicating that fasting affected the anthropometric parameters (PR, SBP, and DBP), mainly in the girls. Salivary antioxidant properties were mostly lower in the girls' fasting group. Therefore, saliva may be a useful stress biomarker during fasting conditions.
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