Microbiological and Biochemical Analysis of Methicillin-resistant Staphylococcus aureus Isolated from Patients Admitted in RIMS, Ranchi
Background: Since methicillin-resistant Staphylococcus aureus strains are resistant to multiple antibiotics, there is a possibility of extensive outbreaks which may be difficult to control. Early detection of methicillin-resistant Staphylococcus is important from patients and hospitals point of view. Materials and Methods: The present study was carried out in the Department of Microbiology, Rajendra Institute of Medical Sciences, Ranchi, clinical isolates of methicillin-resistant S. aureus strains were obtained from admitted patients of Rajendra Institute of Medical Sciences, Ranchi. The sources of isolate included pus from infected surgical wounds, infected burn wounds, conjunctival swab, aural swab, throat swab, vaginal swab, and urine for microbiological and biochemical analysis of methicillin-resistant S. aureus. Results: All the 264 cases of staphylococcal species isolated from different clinical specimens were subjected to coagulase test. It was observed that out of 264 strains of staphylococci isolated from different sites, 165 strains (62.5%) were coagulase positive and 99 strains (37.5%) were coagulase negative by tube method. Out of the 165 strains of coagulase-positive staphylococci, maximum isolation was obtained from pus 74 followed by throat swab 55, aural swab 21, vaginal 4, conjunctival swab 9, and urine 2. All the 165 cases of coagulase-positive Staphylococcus isolated from different clinical specimens were studied for hemolysis, mannitol fermentation, pigment production, and phosphatase production. Out of these 165 strains, 162 (98%) strains produced β-hemolysis on blood agar medium. Pigment production was noted in 160 (97%) of cases. Majority of strains produced characteristic golden yellow pigment on nutrient agar plate. A total of 155 (94%) strains of staphylococci fermented mannitol with the production of acid only. Phosphatase production was observed in 157 (95%) strains of pathogenic staphylococci. Conclusion: Considering the above-mentioned pathogenicity test, it was observed that coagulase test was the single most reliable test, though coagulase-negative staphylococci are sometimes pathogenic too.
Abramson C. Staphylococcal enzymes. In: Cohen JO, editor. The Staphylococci. New York: John Wiley; 1972. p. 187-248.
Taylor TA, Unakal CG. Staphylococcus aureus. Treasure Island, FL: StatPearls Publishing; 2020.
Lakhundi S, Zhang K. Methicillin-resistant Staphylococcus aureus: Molecular characterization, evolution, and epidemiology. Clin Microbiol Rev 2018;31:e00020-18.
Siddiqui AH, Koirala J. Methicillin Resistant Staphylococcus aureus(MRSA) Treasure Island, FL: StatPearls Publishing; 2020.
Karmakar A, Dua P, Ghosh C. Biochemical and molecular analysis of Staphylococcus aureus clinical isolates from hospitalized patients. Can J Infect Dis Med Microbiol 2016;2016:9041636.
Adhikari R, Pant ND, Neupane S, Neupane M, Bhattarai R, Bhatta S, et al. Detection of methicillin resistant Staphylococcus aureus and determination of minimum inhibitory concentration of vancomycin for Staphylococcus aureus isolated from pus/wound swab samples of the patients attending a tertiary care Hospital in Kathmandu, Nepal. Can J Infect Dis Med Microbiol 2017;2017:2191532.
Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH. Manual of Clinical Microbiology. 8th ed. Washington, DC, USA: American Society for Microbiology; 2003.
Raymond EA, Traub WH. Identification of staphylococci isolated from clinical material. Appl Microbiol 1970;19:919-22.
Deepak S, Samant SA, Urhekar AD. Study of coagulase positive and negative staphylococci in clinical samples. Indian J Med Sci 1999;53:425-8.
Majumder D, Bordoloi JN, Phukan AC, Mahanta J. Antimicrobial susceptibility pattern among methicillin resistant Staphylococcus isolates in Assam. Indian J Med Microbiol 2001;19:138-40.
Rajput A, Singh KP, Kumar V, Sexena R, Singh RK. Antibacterial resistance pattern of aerobic bacteria isolates from burn patients in tertiary care hospital. Biomed Res 2008;19:1-4.
Williams RE, Harper GJ. Determination of coagulase and alpha-haemolysin production by staphylococci. Br J Exp Pathol 1946;27:72-81.
Gillespie EH. The routine use of coagulase test for staphylococci. Mon Bull Emerg Public Health Lab Serv 1943;2:19.
Kateete DP, Kimani CN, Katabazi FA, Okeng A, Okee MS, Nanteza A, et al. Identification of Staphylococcus aureus: DNase and mannitol salt agar improve the efficiency of the tube coagulase test. Ann Clin Microbiol Antimicrob 2010;9:23.
Breckinridge JC, Bergdoll MS. Outbreak of food-borne gastroenteritis due to a coagulase-negative enterotoxin-producing staphylococcus. N Engl J Med 1971;284:541-3.
Goyal R, Das S, Mathur M. Colonisation of methicillin resistant Staphylococcus aureus among health care workers in a tertiary care hospital of Delhi. Indian J Med Sci 2002;56:321-4.
Christie R, Keogh EV. Physiological and serological characteristics of staphylococci of human origin. J Pathol Bacteriol 1940;51:189-97.
Abramson C, Friedman H. Enzymatic activity of primary isolates of staphylococci in relation to antibiotic resistance and phage type. J Infect Dis 1967;117:242-8.
Schwabacher H, Cunliffe AC, Willmams RE, Harper GJ. Hyaluronidase production by staphylococci. Br J Exp Pathol 1945;26:124-9.
Barber M. Methicillin-resistant staphylococci. J Clin Pathol 1961;14:385-93.
Barber F, Kuper SW. Identification of Staphylococcus pyogenes by the phosphatase reaction. J Pathol Bacteriol 1951;63:65-8.
Cluff LE, Reynolds RC, Page DL, Breckenridge JL. Staphylococcal bacteremia and altered host resistance. Ann Intern Med 1968;69:859-73.
Copyright (c) 2020 Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Asian Pacific Journal of Health Sciences applies the Creative Commons Attribution (CC-BY) license to published articles. Under this license, authors retain ownership of the copyright for their content, but they allow anyone to download, reuse, reprint, modify, distribute and/or copy the content as long as the original authors and source are cited. Appropriate attribution can be provided by simply citing the original article.