Susceptibility of field-collected mosquitoes (Culex pipiens) in Northern Tunisia to temephos, an organophosphate insecticide

Authors

  • Ahmed Tabbabi Monastir University, 5019, Monastir, Tunisia
  • Ali Lamari Monastir University, 5019, Monastir, Tunisia
  • Ben Haj Ayed Ahlem Monastir University, 5019, Monastir, Tunisia
  • Raja Ben Cheikh Monastir University, 5019, Monastir, Tunisia
  • Hassen Ben Cheikh Monastir University, 5019, Monastir, Tunisia
  • Jaber Daaboub Ministry of Public Health, 1006, Bab Saadoun, Tunis, Tunisia

DOI:

https://doi.org/10.21276/apjhs.2017.4.2.26

Keywords:

Culex pipiens, temephos resistance, detoxification mechanisms, target site alteration, Northern Tunisia

Abstract

In order to understand the resistance of Culex pipiens to temephos and provide parameters for management programs, we evaluated the susceptibility levels to temephos of individuals collected in five localities of Northern Tunisia. Our results showed that sample # 2 was susceptible. The resistant samples displayed RR50 ranged from 1.3 in sample # 5 to 440 in sample # 4. Mortality caused by propoxur ranged from 0% in sample # 4, which showed the
highest resistance levels to studied temephos insecticide and indicated an important contribution of AChE 1, to 68% in sample # 5. Starch gel electrophoresis identified many esterases in studied samples with an important frequency (85%) in the sample # 4. This sample showed the highest resistance to temephos with a major contribution of CYP450, esterases, and AChE 1. Both detoxification mechanisms and target site alteration were involved in the resistance to temephos as reported in our study. This is not a new phenomenon in mosquitoes, in which multiple insecticide resistance mechanisms has been reported worldwide.

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Author Biographies

Ahmed Tabbabi, Monastir University, 5019, Monastir, Tunisia

Laboratory of Genetics, Faculty of Medicine of Monastir 

Ali Lamari, Monastir University, 5019, Monastir, Tunisia

Laboratory of Genetics, Faculty of Medicine of Monastir 

Ben Haj Ayed Ahlem, Monastir University, 5019, Monastir, Tunisia

Laboratory of Genetics, Faculty of Medicine of Monastir

Raja Ben Cheikh, Monastir University, 5019, Monastir, Tunisia

Laboratory of Genetics, Faculty of Medicine of Monastir

Hassen Ben Cheikh, Monastir University, 5019, Monastir, Tunisia

Laboratory of Genetics, Faculty of Medicine of Monastir

Jaber Daaboub, Ministry of Public Health, 1006, Bab Saadoun, Tunis, Tunisia

Department of Hygiene and Environmental Protection

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Published

2017-06-30

How to Cite

Ahmed Tabbabi, Ali Lamari, Ben Haj Ayed Ahlem, Raja Ben Cheikh, Hassen Ben Cheikh, & Jaber Daaboub. (2017). Susceptibility of field-collected mosquitoes (Culex pipiens) in Northern Tunisia to temephos, an organophosphate insecticide. Asian Pacific Journal of Health Sciences, 4(2), 157–161. https://doi.org/10.21276/apjhs.2017.4.2.26

Issue

Vol. 4 No. 2 (2017): Vol 4 | Issue 2 | April - June 2017

Section

Table of Contents

License

Asian Pacific Journal of Health Sciences applies the Creative Commons Attribution (CC-BY) license to published articles. Under this license, authors retain ownership of the copyright for their content, but they allow anyone to download, reuse, reprint, modify, distribute and/or copy the content as long as the original authors and source are cited. Appropriate attribution can be provided by simply citing the original article.