Effect of ultrasound irradiation on some Biophysical Parameters of Human Blood (in vitro)

Abstract

Ultrasound is commonly employed in clinical practice as a part of physical therapy. In this study, an attempt has been made to investigate the effect of ultrasound irradiation on some Biophysical Parameters (pH, Conductivity, and Refractive index) of human blood. Blood samples of volume 5 ml were collected from the donors each time an experiment was performed. Blood was then transferred to heparin anticoagulant tubes. The human blood samples were divided into two parts, that is, control sample and sample for irradiation. The human blood sample was irradiated using an ultrasonic interferometer of 3MHz frequency for a duration of 1 h in an identical and controlled environment. The biophysical parameter was determined and compared with that of the control sample. Variations were observed in the values of pH, conductivity, and refractive index of ultrasonically irradiated human blood sample compared with that of the control sample. This study gives us an idea regarding the effect of ultrasound on some of the most important biophysical parameters of human blood. Further, the results obtained in the present study can be utilized in the future for the work on the biological effects of ultrasound and interpret the in vivo effects.