Detection of Colistin Resistance in Uropathogenic Carbapenem Resistant Klebsiella pneumoniae

Authors

  • Ishrath Razia Riaz Department of Microbiology, Dr. ALM PG IBMS, University of Madras, Taramani, Chennai, Tamil Nadu, India
  • Anusha Hindupur Laboratory Division, National institute of Epidemiology, Indian council of medical research, Ayapakkam, Chennai, Tamil Nadu, India
  • Thota Akhil Raj Department of Microbiology, Dr. ALM PG IBMS, University of Madras, Taramani, Chennai, Tamil Nadu, India
  • Iswarya Anbazhagan Department of Microbiology, Dr. ALM PG IBMS, University of Madras, Taramani, Chennai, Tamil Nadu, India
  • Prabu Dhandapani Department of Microbiology, Dr. ALM PG IBMS, University of Madras, Taramani, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.21276/apjhs.2022.9.1.45

Keywords:

Carbapenem resistance, Colistin resistance, Klebsiella pneumoniae, Urinary tract infection

Abstract

Background: Colistin resistance among carbapenem-resistant Klebsiella pneumoniae (CRKp) strains at an accelerated rate poses a serious global threat which limits therapeutic options. Objective: In this study, 203 laboratory-archived uropathogenic K. pneumoniae isolates were screened for carbapenem and colistin resistance. Methods: Carbapenam resistance screening was performed using combined disc test. Colistin resistance was determined for CRKp isolates, according to clinical and laboratory standards institute- European Committee on Antimicrobial Susceptibility Testing guidelines. Polymerase Chain Reaction for carbapenamase genes (blaNDM-1, blaKPC-2 and blaOXA-1) and plasmid-mediated colistin resistance genes (mcr-1-2) were performed for the resistant isolates. Results: About 30% of the CRKp isolates were resistant to colistin; 57.1% and 35.7% of the isolates carried blaNDM-1 and blaOXA-1 gene, respectively. None of the isolates showed positive for blaKPC-2 and mcr (1-2) genes. Conclusion: Colistin resistance in the absence of a plasmid borne transfer mechanism was observed among our CRKp strains. Escalating colistin resistance is a crucial obstacle in treatment of K. pneumoniae infections and repurposing their usage is important to combat and control the resistance.

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Published

2022-01-15

How to Cite

Ishrath Razia Riaz, Anusha Hindupur, Thota Akhil Raj, Iswarya Anbazhagan, & Prabu Dhandapani. (2022). Detection of Colistin Resistance in Uropathogenic Carbapenem Resistant Klebsiella pneumoniae. Asian Pacific Journal of Health Sciences, 9(1), 156–157. https://doi.org/10.21276/apjhs.2022.9.1.45