Green Synthesis and Characterization of Silver Nanoparticles from Azadirachta indica Leaf Extract and its Antibacterial Study

Authors

  • R. Cibi Department of Biotechnology, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, India
  • N. S. Devika Department of Biotechnology, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, India
  • S. Kavya Sagar Department of Biotechnology, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, India
  • S. Athulya Department of Biotechnology, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, India
  • M. Akhila Balachandran Department of Biotechnology, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, India
  • Aleesha Roy Department of Biotechnology, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, India

DOI:

https://doi.org/10.21276/apjhs.2021.8.4.39

Keywords:

Antibacterial activity, Azadirachta indica, Characterization, Green synthesis

Abstract

Nanotechnology is the emerging branch in the field of science. The current study was conducted on plant-derived material for the green synthesis of silver nanoparticles (AgNPs) using neem leaf extract. The flavonoid and terpenoids found in the extract behaved as a reducing and capping agent. Here, the formation of NPs occurred by the bioreduction of Ag salts into AgNPs by the addition of leaf extract. The reduction was validated by the change in color that is from pale green to brown. The ion to NP reduction occurred due to the natural reducing agent in the neem leaf extract. The functional group presents in the extract and AgNPs are investigated using Fourier transform infrared. The synthesized NPs have undergone characterization by ultraviolet spectrometry between 300 and 700 nm. The peak was spotted at 308 nm. Antibacterial properties of AgNPs were studied against Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. From these results, it can be effectively reported that plant extract can be used in the synthesis of AgNPs and could be used for better results in future in the field of medicine, nano-related works, and so on.

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Published

2022-01-16

How to Cite

R. Cibi, N. S. Devika, S. Kavya Sagar, S. Athulya, M. Akhila Balachandran, & Aleesha Roy. (2022). Green Synthesis and Characterization of Silver Nanoparticles from Azadirachta indica Leaf Extract and its Antibacterial Study. Asian Pacific Journal of Health Sciences, 8(4), 194–198. https://doi.org/10.21276/apjhs.2021.8.4.39