Investigation of Flavonoids in Stereospermum suaveolens DC Leaves (Patala) Using HPTLC Analysis for Inflammatory Pain, Swelling, and Edema Treatment

Abstract

Background: As per extensive research study, it was observed that Stereospermum suaveolens DC (Patala) is rich sources of flavonoids. These phytoconstituents have played an important role in prevention and maintenance of acute and chronic diseases of pain and inflammation. There is need to explore method for identification and quantification of the presence of flavonoids in S. suaveolens DC leaves (SSL) by qualitatively and quantitatively and to establish its mechanism in pain, swelling and edema Aim: The aim of the study was to identify and assess role of flavonoids in SSL by high-performance thin-layer chromatography (HPTLC) analysis and animal screening models of inflammatory pain, swelling, and edema. Materials and Methods: HPTLC to quantify flavonoid in the ethyl acetate fraction of plant component in comparison with Quarcetin. The inflammatory pain, swelling, and edema method were investigated by acetic acid induced writhing method in mice, histamine-induced edema in Wistar rats, and croton oil-induced ear edema in mice. The test doses 125 mg/kg, 250 mg/kg, and 500 mg/kg oral administration (p.o.) of ethyl acetate fraction of SSL were selected by oral acute toxicity OECD 423. Results: HPTLC analytical method estimated presence of flavonoids and estimated amount was 22.64%. However, inhibition in histamine-induced paw edema and reduction inhibition in ear edema significantly (P < 0.05) observed by dose-dependent effect and antinociceptive activity detected on acetic acid induced writhing response method in mild to moderately using one-way analysis of variance method. Conclusion: It was clear that flavonoids were responsible for direct and indirect release of intermediate inflammatory mediators and promote its role in the treatment of pain and swelling.