Essential oils analysis of pseudobulbs of Crepidium acuminatum (D.DON) SZLACH by GC-MS

Abstract

Objective: To analyse Essential oils of Crepidium acuminatum (D.Don) Szlach. Methods: DCM Extracts were prepared for Essential oil analysis. GC-MS was used for perusal. Results: In the present study, 6 phytoconstituents have been identified from the DCM pseudobulbs extract of Crepidium acuminatum D. Don Szlach by GC-MS analysis. The results showed that DCM extract contains mainly essential oils which are caryophyllene 74.89% ; Eugenol 5.56%; Humulene 5.48% ; phenol, 2,4 bis (1,1 dimethylethyl) 5.57%; Caryophyllene oxide 5.33%; 2,5 Octadecadiynoic acid, methyl ester 3.16%. Analysis and identification of the presence of the compound in the extract were done by using the database of the National Institute of Standards and Technology (NIST) library. Conclusions: Out of various compounds analysed Eugenol has already been reported whereas Caryophyllene, Caryophyllene oxide, Humulene, Phenol, 2,4bis ( 1, 1dimethylethyl) and 2,5 Octadecadiynoic acid, methyl ester are being reported first time. These compounds have immense medicinal potential. Thus, it found to possess significant phytonutrients, which attribute to its medicinal worth.