Qualitative and Quantitative Phytochemical Analysis in leaf Extracts of Various Clones of Neolamarckia cadamba (Roxb.) Bosser

Abstract

As sources of natural products, medicinal plants bear a great interest for researchers in recent decades and this interest has increased considerably in finding naturally occurring phytochemicals. Neolamarckia cadamba (Roxb.) Bosser is an important member of indigenous medicinal flora and used traditionally for the prevention of numerous diseases. The aim of the present work was focused to investigate and compare the phytochemical screening such as primary and secondary metabolites such as alkaloids, flavonoids, tannins, saponins, quinones, sterols, phenols, anthocyanin, proteins, carbohydrates, amino acid, terpenoids, steroids, glycosides, and quantification (total phenolics, tannin, and flavonoids) which were done. Phytochemical screening was done for selected 11 N. cadamba clones with different organic solvents (petroleum ether, ethanol, and hot water). The collected leaf samples were processed. Maximum number of nine secondary metabolites was obtained in the Clone No C.S, 13, 15, 42, 43, 60, 80, and 105. Rest of the clones have eight secondary metabolites. The maximum amount of total phenolic (200.7 mg Gallic Acid Equivalents [GAE]/g extract), tannin (196.26 mg GAE/g extract), and flavonoid contents (195.36 mg QE/g extract) were observed in Clone No 60. Hence, leaves of N. cadamba can be considered as a potential source of modern phytochemicals of medicinal importance for further studies.