Association of COL1A2 gene polymorphism with dental fluorosis in children of Jaipur district of Rajasthan, India

Abstract

In India major districts are affected through dental fluorosis. In the Rajasthan, almost all the districts are affected from the fluorosis due to excess ingestion of fluoride through drinking water. Fluoride content calculated in the drinking water and blood through fluoride ion specific electrode method. A case-control study was conducted in two blocks (Jamwaramgarh, Amber) of Jaipur district of Rajasthan, India to investigate the COL1A2 gene polymorphisms with relation to dental fluorosis in Indian children (6-14 years). COL1A2 gene polymorphisms were genotyped using polymerase chain reaction-restriction fragment length polymorphism analysis. Two sites (rs2621208, rs414408) were analyzed for the polymorphism. PCR performed for respective amplicon size for RsaI (865bp) and PvuII (401bp). For the rs414408, PCR product (401bp) was digested with Pvu II enzyme and band pattern was C/C for 240 and 161 bp band, A/C/T for 401,240 and 161 bp band, A/A, T/T 401 bp band. In the control sample pattern type is A/C/T. In the fluorosis affected patients sample band pattern is C/C, A/C/T and C/C. For the rs2621208 site, PCR product (865 bp) was digested with Rsa-I enzyme and expected band pattern was C/C for 412,301,128 and 24 bp band, G/C for 412, 301,152, 128 and 24 bp band, G/G 412, 301 and 152 bp band. In the control sample pattern type is A/C/T. In the fluorosis affected patients sample band pattern is C/C, G/C and G/G. An association between dental fluorosis and the COL1A2 gene polymorphism was observed in fluoride-exposed populations of Jaipur district of Rajasthan, India.