COMPARATIVE EVALUATION OF ANTIMICROBIAL EFFICIENCY OF DIODE LASER, SODIUM HYPOCHLORITE AND THEIR SYNERGISTIC EFFECT AGAINST ENTEROCOCCCUSFAECALIS CONTAMINATED ROOT CANALS-AN IN VITRO STUDY

Abstract

Aim: To compare the efficacy of Diode laser irradiation procedure with that of sodium hypochlorite irrigation procedure and their synergistic effect against Enterococcus faecalis- contaminated root canals.
Materials and methods: Forty root canals of extracted central incisor teeth with straight roots were chosen. The canals were enlarged with 40 size K file and randomly divided into four groups of 10 teeth each. After sterilization, all roots will be inoculated with Enterococcus faecalis for 7 days at 37˚C. The first group was used as a control, the second group was irrigated with 5.25% Naocl solution for 5 min, third group was irradiated with 810-nm Diode laser at output power from 2 W for 5*5secs and fourth group was irrigated with 5.25% Naocl followed by irradiation with Diode laser after drying the canal. The disinfecting efficacy of the groups were tested by collecting dentine chips from the inner walls of the canal and counting colony forming units of viable E. faecalis on agar plates. Statistical analysis were done using Anova and Post Hoc tukey test.
Results: Diode laser alone and Diode laser with sodium hypochlorite shows complete elimination of E.faecalis from the root canal.