Ethanol leaves extract of Mangifera indica (L.) exhibits protective, antioxidative, and antidiabetic effects in rats

Abstract

Objective: Many orthodox drugs used for treatment of diabetes have shown reported deleterious side effects. This study investigates the effects of ethanol leaves extract ofMangiferaindica(L) on alloxan-induced diabetic rats as an attempt to investigate herbal remedy. Methods: Male Wistar rats treated for fifteen days with average weight of 150g were randomlyselected into seven groups; 1(controls, received normal saline),2 (Alloxan-induced diabetic rats, 400mg/kg.bw subcutaneously),3 (Diabetic rats treated with extract,100mg/kg.bw),4 ( Diabetic rats treated with glibenclamide,5mg/kg.bw),5 (Diabetic rats treated with combination of extract and glibenclamide),6 ( extract only),7 (glibenclamide only).Biochemical and anti-oxidant indices were determined with standardized methods. Results: Results from indices studied revealed that alloxan-induced diabetic rats showed significant(p<0.05) increases in fasting blood sugar concentrations, white blood cell counts, lymphocytes counts, platelets counts, serum total cholesterol, triglycerides, liver malondialdehyde levels and percentage DNA fragmentation with corresponding decreases in serum total protein concentrations,red blood cell counts,high density lipoprotein concentrations and liver superoxide dismutase and catalase activities. Howeverin diabetic rats treated with extract(Group 3),levels and activities of these markers werereversed significantly and comparativelywith the standard drug (Group 4). Conclusions: These findings indicatepossible anti-oxidative,anti-diabetic,hypoglycemic,hypolipidemic and hepatoprotective effects of the extract and or a synergistic interaction with the standard drug .