Antibacterial Investigation using Spectrophotometric Assay of the Polar Leaf Extracts of Ficus capensis (Moraceae)

  • Achimugu Dickson Musa Department of Biochemistry, Ibrahim Badamasi Babangida University, Lapai, Nigeria
  • Sandra Tombiah Dorgu Department of Biochemistry, Ibrahim Badamasi Babangida University, Lapai, Nigeria
  • Cyril Ogbiko Department of Pure and Applied Chemistry, Faculty of Science, Usmanu Danfodiyo University Sokoto, Sokoto State, Nigeria
  • Jeremiah Ikko Department of Biochemistry, Ibrahim Badamasi Babangida University, Lapai, Nigeria
  • Muhammad Hausa Shuaib Department of Biochemistry, Ibrahim Badamasi Babangida University, Lapai, Nigeria
  • Okwesili Fred Chiletugo Nwodo Drug and Diet Research, University of Mker, Mker, Nigeria
  • Philipp Krastel Centre for Proteomic Chemistry, Novartis Institute for BioMedical Research, Basel, Switzerland
Keywords: Antimicrobial, Defatted extracts, Ficus capensis, Spectrophotometric

Abstract

This study was designed to explore new antibacterial agent(s) from the defatted methanol and water extracts of Ficus capensis. The extracts were obtained using various solvents, namely, cyclohexane, dichloromethane, methanol, and water by serial exhaustive extraction, after which both extracts were challenged with pure clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Acinetobacter baumannii, and Burkolderia cepacia viz-a-viz standard antibiotics of chloramphenicol, gentamicin, and amoxicillin at 25 μg/ml, 50 μg/ml, and 100 μg/ml concentrations using spectrophotometric method. Using the zone of inhibition as an inhibitory parameter, both extracts of F. capensis showed promising antimicrobial activity in a concentration-dependent manner comparable to the standard antibiotics for all tested microorganisms. This research holds promise for the exploration of various potentially active secondary metabolites which would help in developing pharmaceuticals, especially antibacterial drugs, for combating common microbial infections. This validates the use of the plant leaves in the treatment of broad-spectrum microbial infections in ethnomedicine.

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References

Gills LS. Ethnomedical Uses of Plants in Nigeria. Edo State, Nigeria: Uniben Press; 1992. p. 121.

Ayinde BA, Owolabi OJ. Effect of the aqueous extract of Ficus capensisThunb. (Moraceae) leaf on gastrointestinal motility. J Pharmacogn Phytother 2009;1:31-5.

Ahmadu AA, Zezi AU, Yaro AH. Anti-diarrheal activity of the leaf extracts of Daniella oliveri Hutch and Dalz (Fabaceae) and Ficus sycomorus Miq (Moraceae). Afr J Tradit Complement Altern Med 2007;4:524-8.

Oyeleke SB, Dauda BE, Boye OA. Antibacterial activity of Ficus capensis. Afr J Biotechnol 2008;7:1414-7.

Adebayo-Tayo BC, Odeniyi AO. Phytochemical screening and microbial inhibitory activities of Ficus capensis. Afr J Biomed Res 2012;15:35-40.

Umeokoli BO, Onyegbule FA, Gugu TH, Igboeme SO. Evaluation of the erythropoietic and anti-sickling properties of Ficus capensis leaf extract in the treatment of anaemia. Planta Med 2013;79:79-89.

Mpiana PT, Mudogo V, Tshibangu DS, Kitwa EK, Kanangila AB, Lumbu JB, et al. Antisickling activity of anthocyanins from Bombax pentadrum, Ficus capensis, Zizyphus mucronata: Photo degradation effect. J Ethnopharmacol 2008;120:413-8.

Owolabi OJ, Nworgu ZA, Falodun A, Ayinde BA, Nwako CN. Evaluation of tocolytic activity of ethanol extracts of the stem bark of Ficus capensis Thunb. (Moraceae). Acta Pol Pharm Drug Res 2009;66:293-6.

Daikwo OA, Tende JA, Okey SM, Eze ED, Isa AS. The effect of aqueous extract of leaf of Ficus capensis Thunb (Moraceae) on in vivo leukocyte mobilization in Wistar rats. Br J Pharmacol Toxicol 2012;3:110-14.

Ayinde BA, Owolabi OJ, Jesuorobo R. Active ileum relaxant fractions from the leaves of Ficus capensis Thunb (Moraceae). Niger J Pharm Sci 2013;12:1-7.

Ramde-Tiendrebeogo A, Tibiri A, Hilou A, Lomp M, Millogo-Kone H, Nacoulma OG, et al. Antioxidative and antibacterial activities of phenolic compounds from Ficus sur Forssk. and Ficus sycomorus L. (Moraceae): Potential for sickle cell disease treatment in Burkina Faso. Int J Biol Chem Sci 2012;6:328-36.

Gelfand M, Mavi S, Drummond RB, Ndemera B. The Traditional Medicinal Practitioner in Zimbabwe. Gweru: Mambo Press; 1985. p. 411.

Musa DA, Lifebuoy DG, Cyril O, Okwesili FC. Phytochemical and in vitro anti-typhoid properties of leaf, stem and root extracts of Ficus capensis (Moraceae). J Pharm Bioresour 2019;16:165-172.

Ogbiko C, Eboka CJ, Tambuwal AD. Extraction and fractionation of whole black seed plantain (Plantago rugelii Decne) for in-vitroantioxidant, antibacterial and phytochemical screening. J Appl Sci Environ Manage 2018;22:613-8.

Motaleb MA. Selected Medicinal Plants of Chittagong Hill Tracts. Gland, Switzerland: International Union of Conservation of Nature and Natural Resources; 2011. p. 1-3.

Holt JG, Krieg NR, Sneath PA. Bergey’s Manual of Systematic Bacteriology. 9th ed. Baltimore Maryland: Williams & Wilkins Co.; 1994. p. 786.

Chessbrough M. District Laboratory Practice in Tropical Countries. 2nded. London: Cambridge University Press; 2006. p. 21.

Musa AD, Nwodo OF, Ojogbane EB. Phytochemical, antibacterial and toxicity studies of the aqueous extract of Euclayptus camaldulensisDehnh. Asian J Plant Sci Res 2011;1:1-10.

Musa AD, Dorgu S, Markwalder R, Oshe AO, Kanki H, Nwodo OF, et al. In vitro antibacterial activities of crude and partially purified fractions of defatted ethanol extract of Ficus capensis. Biokemistri 2017;29:67-75.

Arthington-Skaggs BA, Lee-Yang W, Ciblak MA, Frade JP, Brandt ME, Rana A, et al. Comparison of visual and spectrophotometric methods of broth microdilution, MIC, End Point determination and evaluation of a sterol quantitation method for in vitro susceptibility testing of fluconazole and itraconazole against trailing and non trailing candida isolates. Antimicrob Agents Chemother 2012;46:2477-81.

Cooper J, Niggli U, Leifert C. Handbook of Organic Food Safety and Quality. Cambridge, England: Woodhead Publishing Limited Abington Hall Abington; 2006. p. 34.

Uzoekwe NM, Mohammed JJ. Phytochemical, proximate and mineral contents of leaves and Bark of Ficus capensis. J Appl Sci Environ Manage 2015;19:633-7

Alli S. Determination of chemical composition of Senna siamea (Cassialeaves). Pak J Nutr 2009;8:119-21.

Mohammed A, Liman ML, Atiku MK. Chemical composition of the methanolic leaf and stem bark extracts of Senna siamea Lam. J Pharmacogn Phytother 2013;5:98-100.

Ahmad-Alizaga SL, Olayanju S. Phytochemical screening of the leaf extracts of Sennasiamea Lam (Pop corn) and its antibacterial activity. Biol Environ Sci J Trop 2007;4:193-5.

Doughari JH, Okafor NB. Antibacterial activity of Senna siamea leaf extracts on Salmonella

typhi. Afr J Microbiol Res 2008;2:42-6.

François MN, Amadou D, Rachid S. Chemical composition and biological activities of Ficuscapensis leaves extracts. J Natl Prod 2010;3:147-60.

Solomon-Wisdom GO, Shittu GA, Agboola YA. Antimicrobial and phytochemical screening activities of Ficus sur (Forssk). N Y Sci J 2011;4:15-8.

Published
2020-08-05
How to Cite
Musa, A. D., Dorgu, S. T., Ogbiko, C., Ikko, J., Shuaib, M. H., Nwodo, O. F. C., & Krastel, P. (2020). Antibacterial Investigation using Spectrophotometric Assay of the Polar Leaf Extracts of Ficus capensis (Moraceae). Asian Pacific Journal of Health Sciences, 7(3), 23-26. https://doi.org/10.21276/apjhs.2020.7.3.6