Effect of different irrigation regimens on smear layer removal in human root dentin an invitro study

Abstract

Introduction: Aim of the study is to compare the efficacy of 5% NaOCl with 17% EDTA, 18% Etidronic Acid, 9% Etidronic Acid and 0.2% Chitosan as different protocols of irrigating solutions for smear layer removal using scanning electron microscope. Methodology: Forty extracted human teeth were collected and stored in saline. Single rooted teeth with complete, mature root apices without any anatomic variation having straight patent root canal extracted for periodontal cause, were included in the present study. Teeth where then divided into four groups with 10 teeth in each group. Access opening followed by Bio-mechanical Preparation was done and teeth were irrigated as follows (n=10 per group).Group 1: 5% NaOCl during instrumentation, 17% EDTA after instrumentation (3min),Group 2: 5% NaOCl during instrumentation, 9% Etidronic acid after instrumentation (5min),Group 3: 5% NaOCl during instrumentation, 18% Etidronic acid after instrumentation (3min),Group 4: 5% NaOCl during instrumentation, 0.2% Chitosan solution after instrumentation (5min).After the irrigation of specimens longitudinal sectioning of specimens was done and subsequently smear layer removal ability will be evaluated.Results: All irrigants tested, removed smear layer effectively form the apical third. EDTA (Group 1) showed comparatively better results than 9% Etidronic Acid (Group 2), 18% Etidronic Acid (Group 3) and 0.2% Chitosan (Group 4) at the apical third. Conclusion: There is no significant difference between 17% EDTA, 9% Etidronic Acid, 18% Etidronic Acid, and 0.2% Chitosan in the ability to remove smear layer.