Stability indicating simultaneous validation of hydrochlorothiazide, dihydralazine, and propranolol with thestudy of forced degradation behavior through RP-HPLC in thepharmaceutical dosage form

Authors

  • Akhilesh Sharma Pacific Academy of Higher Education and Research University, Udaipur(Rajasthan), India
  • Anurag Mishra Pacific Academy of Higher Education and Research University, Udaipur(Rajasthan), India
  • Sanjay Sharma SVKM’S, NarseeMonjee Institute of Management Studies,Mumbai-Agra Road, Shirpur-425405, India

DOI:

https://doi.org/10.21276/apjhs.2017.4.3.44

Keywords:

HPLC, Hydrochlorothiazide, Dihydralazine, Propranolol, Validation, Forced degradation

Abstract

For the instantaneous assay of Hydrochlorothiazide, Dihydralazine and Propranolol in tablet, an easy, accurate and truthful RP-HPLC method have been developed as well as validated. Isocratic RP-HPLC method was developed on BDS hypersil C18, (250mm×4.6mm internal diameter, 5µ particle size) using mobile phase as 0.05M Potassium Dihydrogenortho Phosphate (pH-6.0): Acetonitrile (40:60v/v) at a 1.0 mL/min flow rate than at 215 nm detection was carried out using tunable absorbance detector (Waters 486). Study of forced degradation study was carried out through acid degradation, base degradation, thermal degradation, oxidation of the drug. The validation of method was carried out to observe accuracy, precision, linearity, in addition to robustness. The method was found to be linear in the concentration range of 10-30

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Author Biographies

Akhilesh Sharma, Pacific Academy of Higher Education and Research University, Udaipur(Rajasthan), India

Faculty of Pharmaceutical Sciences, 

Anurag Mishra, Pacific Academy of Higher Education and Research University, Udaipur(Rajasthan), India

Faculty of Pharmaceutical Sciences,

Sanjay Sharma, SVKM’S, NarseeMonjee Institute of Management Studies,Mumbai-Agra Road, Shirpur-425405, India

School of Pharmacy and Technology Management, 

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Published

2017-09-30

How to Cite

Akhilesh Sharma, Anurag Mishra, & Sanjay Sharma. (2017). Stability indicating simultaneous validation of hydrochlorothiazide, dihydralazine, and propranolol with thestudy of forced degradation behavior through RP-HPLC in thepharmaceutical dosage form. Asian Pacific Journal of Health Sciences, 4(3), 292–305. https://doi.org/10.21276/apjhs.2017.4.3.44

Issue

Vol. 4 No. 3 (2017): Vol 4 | Issue 3 | July - September 2017

Section

Table of Contents

License

Asian Pacific Journal of Health Sciences applies the Creative Commons Attribution (CC-BY) license to published articles. Under this license, authors retain ownership of the copyright for their content, but they allow anyone to download, reuse, reprint, modify, distribute and/or copy the content as long as the original authors and source are cited. Appropriate attribution can be provided by simply citing the original article.