RP-HPLC Method Development and Validation for Simultaneous Estimation of Alprazolam and Melatonin in Pharmaceutical Dosage Form
Keywords:Method validation, RP-HPLC, Alprazolam, Melatonin, Dosage form
Melatonin a hormone is a major product of the pineal gland. Alprazolam is an anti-anxiety drug that belongs to the benzodiazepines category. The quantity of alprazolam and melatonin had been calculated by the use of a regression equation and the responses were measured at 277 nanometers wavelength using the above-mentioned conditions RP-HPLC simultaneous estimation of Alprazolam and Melatonin has developed a method and determined for validation. The method was estimated as specific and no interference was seen from impurities and excipients. The present study method developed as simple, precise, and accurate for determination and can be further used for the analysis of Alprazolam and Melatonin for dosage formulation.
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Copyright (c) 2022 Rupsi, Rakesh Kumar
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