RP-HPLC Method Development and Validation for Simultaneous Estimation of Alprazolam and Melatonin in Pharmaceutical Dosage Form

Authors

  • Rupsi Department of Chemistry, Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu Rajasthan, India.
  • Rakesh Kumar Department of Chemistry, Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu Rajasthan, India.

DOI:

https://doi.org/10.21276/apjhs.2022.9.4.57

Keywords:

Method validation, RP-HPLC, Alprazolam, Melatonin, Dosage form

Abstract

Melatonin a hormone is a major product of the pineal gland. Alprazolam is an anti-anxiety drug that belongs to the benzodiazepines category. The quantity of alprazolam and melatonin had been calculated by the use of a regression equation and the responses were measured at 277 nanometers wavelength using the above-mentioned conditions RP-HPLC simultaneous estimation of Alprazolam and Melatonin has developed a method and determined for validation. The method was estimated as specific and no interference was seen from impurities and excipients. The present study method developed as simple, precise, and accurate for determination and can be further used for the analysis of Alprazolam and Melatonin for dosage formulation.

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Published

2022-06-20

How to Cite

Rupsi, & Rakesh Kumar. (2022). RP-HPLC Method Development and Validation for Simultaneous Estimation of Alprazolam and Melatonin in Pharmaceutical Dosage Form. Asian Pacific Journal of Health Sciences, 9(4), 309–311. https://doi.org/10.21276/apjhs.2022.9.4.57

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