To Evaluate In Vitro Acetylcholinesterase Inhibitory Activity of Various Indian Medicinal Plants
Keywords:Autographic assay, Rf value, 2, 2-diphenyl-1-picrylhydryzyl method, Acetylcholinesterase inhibitory activity, Antioxidant, Microplate assay
Medicinal herbs are active in curing various disorders by their distinct characteristics. In this research, antioxidant and acetylcholinesterase (AChEI) inhibitory activity of Achyranthes aspera, Psidium guajava, Anthocephalus cadamba, Carissa carandas, Caesalpinia bonduc, Indian medicinal plants have been evaluated by collecting and extracting selected plants parts by using different solvents followed by assessment of AChEI inhibitory activity by autographic assay (TLC method) and microplate assay (Ellman method). Further assessment of antioxidant activity of selected plants by 2, 2-diphenyl-1-picrylhydryzyl method was done. In autographic assay, C. bonduc had more AChE inhibitory potential than other selected plants. In microplate assay method, C. bonduc has shown lowest IC50 value among all other extracts, suggesting its highest enzymatic inhibitory potential and C. carandas has been shown highest IC50 value, suggesting a lower enzymatic inhibitory potential. The Rf value of C. bonduc, A. aspera, P. guajava, A. cadamba, and C. carandas was found to be 0.71, 0.5, 0.3, 0.45 and 0.2, respectively. From the results, leaves of P. guajava showed good antioxidant potential and C. bonduc seeds showed maximum cholinesterase inhibitory activity.
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Copyright (c) 2022 Shivani Singh, Ashu Sapra, Saloni Kakkar, Meenu Bhan, Ashwani Kumar Jangra
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